人牙龈结合上皮、口腔龈上皮及沟内上皮中细胞角蛋白的表达

Cytokeratin expression in human junctional epithelium, oral epithelium and sulcular epithelium

目的检测人牙龈上皮的细胞角蛋白(CK)谱,探讨结合上皮(JE)与口腔龈上皮(OE)、沟内上皮(SE)的不同。方法5例人磨牙及前磨牙牙龈颊舌向石蜡切片,采用免疫组化SP法行CK5/6、7、8/18、10/13、16、17、19、20染色。结果CK7、17在3种上皮中均无表达;CK5/6、20在3种上皮的基底上层(尤其是近表层)为弱阳性及阳性表达,基底层无表达;CK10/13、16在JE全层均有表达,在OE和SE仅限于基底上层,其中CK10/13为强阳性,CK16为弱阳性及阳性;CK19在JE全层强阳性,在OE和SE仅限于基底层,JE和SE分界明显;CK8/18与CK19相似,只是表达较弱,近基底层的基底上层也有少量表达。结论JE是一种不同于OE和SE的特殊低分化上皮,CK19可作为JE的特征性标记物区别于OE和SE,CK10/13、16可用于体外培养的上述3种细胞的鉴别。

Objective To investigate the expression of cytokeratins (CK) in the normal human gingival epithelium and to explore the difference between junctional epithelium (JE), oral epithelium (OE) and sulcular epithelium (SE).Methods Teeth specimens with gingival tissue were collected from 5 people. Paraffin-embedded sections were stained with monoclonal antibodies responded respectively to human CK5/6, 7, 8/18, 10/13, 16, 17, 19, 20. Results CK7 and 17 was not expressed in all strata of JE, OE and SE. CK5/6 and 20 were weekly or moderately expressed in the suprabasal, and not expressed in the basal layer of all three epithelia. CK10/13 and 16 were positive in all strata of JE and in the suprabasal layers of OE and SE. CK10/13 was moderately to strongly expressed and CK16 was weekly to moderately expressed. The staining for CK19 was intense in all strata of JE and the basal layer of OE and SE. There was a remarkable demarcation between JE and SE. The pattern of CK8/18 expression was similar to that of CK19, but was weaker. Besides the basal layer, some superbasal layers close to the basal layer were stained. Conclusions JE is an unique non-differentiated stratified epithelium different from OE and SE. CK19 would be a histological marker and CK10/13, 16 would be the cellular markers to differentiate JE from OE and SE.