人直肠腺癌细胞系HRC-99的建立及其生物学特性

Establishment and characterization of a cell line HRC-99 from human rectal adenocarcinoma

目的:建立一人直肠癌细胞系并探讨其生物学特性方法:取人直肠癌新鲜手术标本,经组织块体外培养并克隆建系,命名为HRC-99.采用光镜、电镜、免疫组化、染色体核型分析及异种移植瘤实验对细胞系生物学特性进行观察. 结果:原位肿瘤、细胞系及移植瘤标本经光镜、电镜证实该细胞系具有腺上皮性恶性细胞特征,并观察了其体外生长曲线、倍增时间和分裂指数:免疫组化CEA和CK-20呈阳性表达;染色体核型分析众数为60,为亚三倍体,G显带出现标记染色体和染色体易位;裸鼠皮下异种移植形成移植瘤. 结论:HRC-99为一株新的来源于直肠中分化腺癌的细胞系.

AIM: To establish a cell line from human moderately differentiated rectal adenocarcinoma, and to investigate its biological characteristics. METHODS: Cancer tissues taken from freshly resected specimens of patients with primary rectal adenocarcinoma were incubated in vitro. The cell line, we named HRC-99, was established. The biological characteristics of these cells were studied by light microscopy, electron microscopy, immunohistochemistry, chromosome analysis and hetero-genic transplantation experiment. RESULTS: Cells from specimens of the primary tumor, the HRC-99 cell line and transplanted tumor were confirmed possessing the characteristics of malignant glan-dular epithelium under light and electron microscope. The cell growth curve, duplicating time and cleavage index in vitro were also observed. CEA and CK-20 were positively expressed in the cells. Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 60 per cell, and marker chromosome and chromosome translocation were found in G band as well. Tumor nodes were induced under the skin of nude mice by heterogenic transplantation. CONCLUSION: The HRC-99 cell line is a new cell line out of rectal moderately differentiated adenocarcinoma.