多聚酶链反应技术在斑点热群立克次体流行病学调查中的应用

The Application of PCR to Epidemiological Study on Spotted Fever Group Rickettsiae

作者首次用来自立氏立克次体（Ｒ．ｒｉｃｋｅｔｔｓｉｉ，Ｒｒ）１９０ＫＤａ蛋白抗原基因序列设计的一对引物扩增从黑龙江、河北、海南、北京采集的蜱、蜱卵、幼蜱、蜱粪及啮齿动物脏器中斑点热群立克次体ＤＮＡ。结果从上述标本中均检测出了斑点热群立克次特异的５３２ｂｐ大小的ＤＮＡ片段，该结果部分与同期进行的病原分离结果一致。故认为，ＰＣＲ技术是一种快速、敏感、特异的斑点热群立克次体流行病学调查方法。

It was the first time that a primer pairs derived from the 190KDa protein antigen gene of R. rickettsii were used to amplify SFGR DNA in ticks.ticks. tick ova,larva ,tick faeces and rodent organs which were collected in Hebei,Heilongjiang,Hainan nd Beijing. A 532bp fragment was respectively amplified from above samples.The results were partially in concordance with data obtained through rickettsiae isolation. It was suggestedthat PCR is a rapid,specific,sensitive and practical method for detection of SFGR in endemic foci.