纤维连接蛋白在膀胱癌中的分布及BCG膀胱灌注后的变化

Fibronectin (Fn) distribution in bladder cancer and after intravesical BCG therapy

应用免疫组化技术对３２例膀胱癌，１２例ＢＣＧ灌注前后及１０例正常人膀胱和癌组织中的纤维连接蛋白（Ｆｎ）分布进行了观察。结果显示：正常膀胱组织中Ｆｎ分布于基底膜及粘膜下组织，上皮层无Ｆｎ；膀胱癌中基底膜Ｆｎ有不同程度丧失，分化差，浸润癌中基底膜Ｆｎ丧失明显；ＢＣＧ灌注后，膀胱粘膜中基底膜Ｆｎ裸露，基膜Ｆｎ增多。

To investigate the distribution of Fn in bladder cancer and its change in bladder mucosa after intravesical instillation of BCG,32 specimens of bladder cancer,12 samples of bladder mucosa before and after in stillation of BCG were evaluated by an immuno peroxidase assay with 10 normal cases served as controls. Fn was observed at the basement membrance (BM Fn) and submucosa in the normal bladder tissue, while in bladder cancer,there was different degree of BM Fn loss which was closely related to the degree of tumor differentiation and the depth of tumor invasion,the poorer the tumor differentiation or the deeper the tumor invasion, the more the loss of BM Fn.After intravesical instillation of BCG, a loss of epithelium resulting in an exposure of BM Fn and an apparent incerase of BM Fn were observed in the bladder mucosa.The relationship between Fn distribution and intravesical BCG therapy was discussed.