聚合酶链反应技术诊断泌尿系结核

PCR in the diagosis of urinary tuberculosis

应用聚合酶链反应（ＰＣＲ）技术检测尿中结核杆菌，诊断泌尿系结核；同时对尿标本进行涂片抗酸染色，将病理诊断作为黄金标准，并比较其结果。表明：ＰＣＲ法灵敏度为５０．０％，特异性为８６．０％，两者均高于涂片法（Ｐ＜０．０１）。说明ＰＣＲ检测尿中结核杆菌以诊断泌尿系结核为一先进、可靠、特异、灵敏的诊断手段。对该法临床应用的局限性也进行了初步探讨。

The polymerase chain reaction (PVR) was used to detect M.tuberculosis in urine which was in the meantime detected by acid-fast staining too. The final diagnosis was established on pathological study. The sensitivity of PCR has been 50 %and its specificity 86.0%, both of which were higher than those of acid-fast staining. PCR was chained to be a reliable, specific and sensitive procedure for the diagnosis of urinary tuberculosis.