深低温冷冻保存家兔性腺器官的研究

A study on rabbit's sexual glands frozen at ultra low temperature

自１９９２年７月开始行深低温（－１９６℃）冷冻保存兔性腺研究。冷冻睾丸３６个，１５天后解冻，完好率９１．７％，其中２０个行器官移植，睾丸接通血管均即时建立血循环。４只兔的冷冻睾丸自体移植后，每次采精７４０．６±１８９０．４万个。２只兔的冷冻睾丸异体移植后，每次采精６３．４±３７．７万个。冷冻兔卵巢１４个，１５天后解冻，完好率９２．９％，其中８个行器官移植，卵巢接通血管均即时建立血循环，术后雌二醇水平为２．７２～１２７．３ｐｍｏｌ／Ｌ。冷冻睾丸及卵巢移植一段时间后病理切片证实睾丸及卵巢组织存活。

Since July 1992,we made a study on the preservation of rabbit's sexual glands frozen at ultra low temperature(-196℃).36 testes were frozen for lo days and then rewarmed.The intact rate of the testes has been 91.7% 20 of them were transplanted immediately as soon as the blood vessels have been anastomosed. Autotransplantion of the frozen testes was carried out in 4 rabbits and allotransplanted in 2.The sperm count per semen collection was 7.406±18.904 million and 0.634±0.377 million respectively.14 rabbit's ovaries were likewise frozen and rewarmed and the intact rate was 92.9%.8 ovaries were transplanted and the blood circulation has been restablished promptly.the estradiol level the host rabbits was 2.4￣34.7pg/ml.Pathological study of the transplanted frozen testes and ovaries revealed that the studied tissues were healthy and alive.