P53突变蛋白在膀胱癌及癌旁组织中的表达

Expression of mutant P53 gene in bladder carcinoma and adjacent bladder mucosa

应用ＡＢＣ免疫组化方法及核仁组成区（ＮＯＲｓ）染色对５５例手术切除的膀胱癌及癌旁膀胱粘膜组织石蜡包埋标本进行了Ｐ５３突变蛋白表达的检测及ＡｇＮＯＲ颗粒计数。结果：５５例膀胱癌标本中３３例Ｐ５３突变蛋白高表达，阳性率为６０．０％；５５例癌旁粘膜中１３例Ｐ５３蛋白弱表达。伴有盆腔淋巴结转移的２３例膀胱癌标本中２０例（８７．０％）Ｐ５３蛋白高表达，１１例癌旁组织弱表达。Ｐ５３表达阳性的膀胱癌组织中，每核ＡｇＮＯＲ颗粒平均数（９．５１±１．８７）较Ｐ５３表达阴性者（６．７１±１．６８）明显增多（Ｐ＜０．０１）。结果提示：抑癌基因Ｐ５３的突变是肿瘤增生异常活跃的因素之一，在膀胱癌的发生中是一种比较常见的基因突变，与膀胱癌的浸润和转移能力密切相关。

With ABC immunohistochemical technique,the expression of P53 was studied in paraffin sections of the tumor tissue and the adjacent mucosa resected from 55 patients with bladder cancer.P53 was found to be positive in the nuclei with extensive staining in 33(60.0%),the P53 positive cells being distributed diffusedly in the cancer tissue. 23 adjacent mucosa specimens were weakly stained.In cancer patients with metaplasia in the pelvic lymph nodes,P53 was positive in 20 tumor specimens out of 23(87.0%).AgNORs was also studied with silver staining technique.The number of AgNOR dots per nucleus detected in bladder cancer sections with positive p53staining(9.5±2.87) was greater than those with negative staining(6.7±1.68).The difference was statistically significant(P<0.01).The result suggested that P53 mutation might be one of the genetic events in the development of bladder cancer and might play an important role in tumor invasion and metastasis.