ACE基因多态性与NIDDM伴心血管疾病的相关性

CORRELATION BETWEEN POLYMORPHISM OF ACE GENE AND NIDDM WITH CARDIOVASCULAR DISEASES

以血管紧张素Ⅰ转化酶（ＡＣＥ）基因为候选基因，随机挑选１３５例ＮＩＤＤＭ患者和５７例正常人，用ＰＣＲ方法检测ＡＣＥ基因的缺失／插入多态性。结果发现研究人群的ＡＣＥ基因型分布趋势与法国白种人群一致，而与日本人群的分布趋势显著不同。ＡＣＥ基因的几种基因型均与ＮＩＤＤＭ伴发高血压无相关关系。但Ｄ等位基因与ＮＩＤＤＭ患者具有典型心肌缺血的异常心电图表现显著相关，Ｄ／Ｄ基因型在该组有异常心电图表现的ＮＩＤＤＭ患者中频率明显增高，提示携带该基因型的患者具有冠心病易感性。在ＮＩＤＤＭ患者中进行ＡＣＥ基因型检查有助于对冠心病的早期诊断和治疗。

Diabetes mellitus,hypertension and coronary heart disease (CHD)are polygenetic diseases which have common genetic back-ground.The incidence of cardiovascular diseases ill diabetes is higher than that in general population.Our study takes the an-giotensin Ⅰconverting enzyme gene as a candidate gene,and detect the deletion/insertion polymorphism of ACE gene by PCRmethed in 135 NIDDM patients and 57 controls.We found the distribution of genotypes in our population is the same as that ofCaucasian population, but is different from Japanese population. There is no association between the polymorphism of ACE geneand hypertension in NIDDM patients in our study. We found that the D allele is associated with abnormal EKG in NIDDM patients. The frequency of DD genotype in this group is higher than that in patients without abnormal EKG. So this result shows that NID-DM patients whose genotypes are DD have the susceptibility for CHD. That this polymorphism is of interest in primary preventionand detection of CHD in NIDDM,particular for patients carring the DD genotype.