胃癌变过程ras基因活化和DNA含量的综合研究

MULTIPLE ANALYSIS FOR THE C-HA-RAS ACTIVATION AND DNA CONTENT IH HUMAN GAS-TRIC PRECANCEROUS LESSIONS

为了解胃癌变过程中ｒａｓ基因活化、ｐ２１表达和ＤＮＡ含量的关系，应用分子生物学技术及流式细胞术对６６例胃癌及癌前组织进行了ｃ－Ｈａ－ｒａｓ第１２位密码子点突变、ｐ２１表达和ＤＮＡ含量的综合分析，并进行了４２～６０个月的随访观察。研究发现：ｐ２１放大表达提供胃癌变的较早期信息；ｃ－Ｈａ－ｒａｓ基因活化发生于胃癌变的较早期阶段并提示胃癌预后不良；ＤＮＡ非整倍体的出现起到协助临床病理诊断胃癌的作用，用于早期诊断的价值似乎有限。提示ｃ－Ｈａ－ｒａｓ活化及ｐ２１放大表达可能有助于临床胃癌的早期诊断。

The relationship between ras activation and over expression of p21 protein and DNA content in pro-gression from benign to malignant of gastric mucosa was studied. The point mutation at 12 of c-Ha-rasoncogene was detected with PCR and specific oligonucleotide probe hybridization, the expression of ras p21and DNA ploidy by flow cytometry was also carried out simultaneously on 66 human gastric tissues withgastric cancer and precancerous lessions,some of the patients were followed up for 42 to 60 months, Theresults showed that:activation of ras oncogene and p21 overexpression may be involved in the early stagesof gastric cancer; DNA aneuploidy may not help for the early diagnosis, but can be useful for the diagnosisof gastric cancer in pathology; gastric cancer with mutational activation at 12 of c-Ha-ras may has un-favourable future, It concluded that c-Ha-ras activation and p21 overexpression may be help to early diag-nosis of gastric cancer in clinic.