地方性砷中毒皮损表皮细胞核DNA含量的流式细胞分析

Analysis of Nuclear DNA Content of Epidermal Cells of Skin Lesion in Endemic Arsenism by Flow Cytometry

流式细胞计数仪对地方性砷中毒患者的掌跖角化、躯干色素异常以及皮肤癌细胞核的DNA含量进行了定量分析,并与正常作了对照。检测结果表明,地方性砷中毒患者的不同损害DNA指数与正常(1.0±0.02)相比,有显著性差异(P<0.01,P<0.05)。掌跖角化过度的DNA指数、细胞周期百分率与皮肤癌之间无显著性差异。提示砷性角化病易发生癌变,推测这两种皮损的表皮细胞的功能活动和细胞动力学信息有共同之处。

This article reports the results of the quantitative analysis for the DNA content of cell nucleus of the skin lesion in patients with endemic arsenism by using the technique of flow cytometry, the skin lesions include palmoplantar hyperkeratosis, abnormal pigmentation on trunk and skin cancers. Our results showed that the DNA indices of the different skin lesions of patients in endemic arsenism were significantly higher than those of controls(P<0.01, P<0.05), but there was no significant difference in the DNA index and the cell cycle percentage between the palmoplantar hyperkeratosis and the skin cancer. It is suggested that arsenical keratosis develops easily into skin cancer. It is our opinion that there may be a similarity between the functional activity of epidermal ceils of above two lesions and the information of cell dynamics.