银屑病患者HLA-DR基因分型的研究

HLA-DR Genotyping Using Polymerase Chain Reaction and Sequence-Specific Oligonucleotide Probes in Patients with Psoriasis

利用多聚酶链反应技术及序列特异性寡核苷酸探针对３０例银屑病患者进行了ＨＬＡ－ＤＲ基因分型，并与２５５例北方汉族正常人ＨＬＡ－ＤＲ基因频率进行了比较。发现ＨＬＡ－ＤＲ７基因频率在银屑病患者较正常对照组明显增高（２５．０：１４．５，Ｐｃ＜０．０５，ＲＲ＝２．４４６），而ＨＬＡ－ＤＲ９基因频率却较正常对照组明显降低（３．３３： １２．４，Ｐｃ＜０．０５，ＲＲ＝０．２１８）。提示银屑病致病基因与ＨＬＡ－ＤＲ７关联。

HLA-DR gene frequency was obtained using polymerase chain reaction (PCR) and sequence- specific oligonucleotide (SSO) probes in 30 patients with psoriasis. Comparison of the HLA-DR gene frequency between the 30 patients and 255 normal controls showed that: HLA-DR7 gene frequency was significantly increased in the patients (25. 0 vs 14. 5, Pc 0. 05,RR = 2. 446), while HLA-DR9 gene frequency was obviously decreased in the patients (3. 33 vs 12. 4, Pc<0. 05, RR^0. 218). The results showed that the psoriatic pathogenic gene might be closely related to the HLA-DR7 alleles.