系统性红斑狼疮纤溶活性的实验研究　

Study of Fibrinolytic Activity in BXSB Mice with Lupus Nephritis

采用发色底物显色法和酶联免疫吸附法测定了系统性红斑狼疮（ＳＬＥ）动物模型ＢＸＳＢ小鼠发病前后血中组织型纤维蛋白溶解酶原激活物（ｔ－ＰＡ）和ｔ－ＰＡ抑制物（ＰＡＩ）的含量及活性，同时观察了肾组织病理及尿蛋白量的变化。实验结果表明：发病后小鼠尿蛋白量明显增加，肾小球免疫复合物沉积，毛细血管腔闭塞，管壁增厚，纤维蛋白沉积；血中ｔ－ＰＡ活性明显低于未发病小鼠。提示ＳＬＥ血栓形成与血中ｔ－ＰＡ活性低下有关。

In this paper,the activity and quantity of tissue-type plasrninogen activator(t--PA) and inhibitor of t-PA (PAI) in plasma of BXSB mice with lupus nephritis were measured by spectrophotometric assay and enzyme linked immunosorbent assay(EI-ISA). The results showed that the amount of urine protein increased significantly. The immunopathologic and histologic changes of BXSB mice with lupus nephritis showed deposition of immune complex and fibrin in glomeruli,thickening of capillary walls and occlusion of capillaries. The activity of t--PA in the plasma of diseased BXSB mice was significantly lower than that in normal mice (P<0. 01 ). These results suggest that thrombosis in SLE is correlated to the low activity of t--PA.