粘附式细胞仪测试念珠菌DNA荧光强度的研究

Determination of DNA-fluorescence Intensity of Candida Species with Adherent Cell Analysis and Sorting Cytometer

为了探讨ＤＮＡ含量在真菌分类与菌种鉴定中的作用，我们用粘附式细胞仪测定了对数生长期的白念珠菌、热带念珠菌、季也蒙念珠菌细胞内ＤＮＡ荧光强度。结果显示：综合荧光值种间比较，无种间特异性。平均荧光值具有种间特异性，同种不同株的白念珠菌株间比较，无显著性差异。其中，热带念珠菌平均荧光值最高，季也蒙念珠菌则最低。而且，平均荧光值比较稳定，变异小。因此，对真菌平均荧光值的进一步研究，有可能为真菌的分类鉴定提供新的途径。

DNA-fluorescence intensity of Candida isolates including C. albicans, C. tropicalis and C. guilliermondi growing in exponential -growth-phase was determined by adherent cell analysis and sorting cytometer. All specimens were treated with RNAase and stained with propidium idodide(PI). The results showed that interspecific difference in average fluorescence value of exponential-growth-phase was found, the average flourescence value of C. tropicalis was the highest, and that of C. guilliermondi was the lowest. It is suggested that the average fluorescence value, the intensity of DNA fluorescence particle in single cell is a stable and interspecific parameter, which may be used as an useful method for the classification of fungus.