FK506免疫抑制机理的研究

Research on the immunosuppressive mechanism of FK506

通过采用淋巴细胞增殖试验、混合淋巴细胞反应和间接免疫荧光法分析淋巴细胞表面白细胞介素２受体（ＩＬ－２Ｒ）和ＴｆＲ的表达，研究了ＦＫ５０６的免疫抑制作用。结果表明，ＦＫ５０６对植物血凝素（ＰＨＡ）诱导的淋巴细胞增殖具有强烈的抑制作用，５０％抑制浓度（ＩＣ５０）为１．３５ｎｍｏｌ／Ｌ，外源性ＩＬ－２不能逆转这种作用；对混合淋巴细胞反应（ＭＬＲ）有显著抑制作用，其ＩＣ５０为０．１２ｎｍｏｌ／Ｌ；在由ＰＨＡ和ＭＬＲ诱导的淋巴细胞表面ＩＬ－２Ｒ和ＴｆＲ表达中，对ＩＬ－２Ｒ和ＴｆＲ的表达均呈抑制作用。

The effect of FK506 on PHA-and alloantigen-induced human peripheral blood lymphocyte Proliferation,expressions of IL-2R and TfR were investigated by the methods of 3H-TdR incorporation and indirect immunofluorescence respectively. The results demonstrated that FK506 had an inhibitory effect on PHA-induced proliferation (IC50 13.5 nmol/ L), which could not reversed by exogenous recombinant IL-2. In both PHA-stimulants and alloactivated lymphocytes, FK506 could inhibit the expressions of TfR and IL-2R.This study sugguested that FK506 was an effective immunosuppressive agent.