脑肿瘤局部浸润单个核细胞亚群及其免疫功能的原位免疫组织化学观察

In situ immunohistochemical observation on subpopulations and immune function of infiltrating mononuclear cells in human brain tumors

应用免疫组化方法对５１例脑肿瘤局部浸润的单个核细胞进行观察。发现主要是辅助／诱导（ＣＤ４）Ｔ细胞、抑制／细胞毒（ＣＤ８）Ｔ细胞和单核巨噬细胞参与肿瘤免疫。仅９例及７例分别有少许Ｂ细胞与ＮＫ细胞。ＣＤ４、ＣＤ８Ｔ细胞的密度与单核巨噬细胞密度呈正相关，与肿瘤体积呈负相关。各病理类型脑肿瘤中ＣＤ４亚群均受抑制，胶质瘤还选择性抑制ＣＤ８亚群。脑肿瘤局部免疫状态不受肿瘤发生部位和浸润Ｔ细胞标记增殖期淋巴细胞（Ｋｉ－６７）抗原、标记活化Ｔ细胞（ＨＬＡ－Ｄｒ）及标记白细胞介素－２－受体（ＣＤ２５）表达水平的影响。

Abstract The tumor infiltrating MNC were investigated in 51 brain tumors by immunohistochemistry.It was found that most of them were CD4 and CD8 T cells.The densities of CD4 and CD8 T cells were correlated positively with that of macrophases and negatively with the tumor size. The mean value of CD4/CD8 was 0.85.In the gliomas,the density of CD8 T cells was statistically lower than in meningiomas(P<0.05).Only in 9 and 7 cases were a few B cells and NK cells found respectively.These results suggest that CD4,CD8 T cells and macrophages play a major role in immunoresponse against brain tumors and that it is possible all of brain tumors inhibit CD4 subset,gliomas also inhibit CD8 subset selectively,and these tumors and host immune system restrain each other.In addition,the local immune status of brain tumors is not affected by the anatomical site where tumors occur or the level of expression of ki-67,HLA-Dr and CD25 in the tumorinfiltrating T cells.