肾病患儿外周血单个核细胞中白细胞介素1β及其受体拮抗剂的基因表达

IL-1beta, IL-1ra mRNA expression of peripheralblood mononuclear cells (PBMC) in idiopathicnephrotic syndrome (INS) detected by biotin-labelledprobe in situ hybridization

为进一步了解原发性肾病综合征（ＩＮＳ）的发病与细胞免疫功能紊乱的关系，作者应用生物素标记ｃＤＮＡ探计、链霉亲合素－硷性磷酸酶检测系统行细胞涂片原位杂交，并用真彩色医学图像分析系统对杂交信号行定量分析，检测４例ＩＮＳ患儿及５例正常儿外周血单个核细胞（ＰＢＭＣ）中的白细胞介素１β（ＩＬ－１β）及其受体拮抗剂（ＩＬ－ｌｒａ）信使核糖核酸（ｍＲＮＡ）的表达。结果：在患儿ＰＢＭＣ中，ＩＩ－１β和ＩＬ－ｌｒａｍＲＮＡ的表达都明显低于正常对照（１０８±２０比１０６±３１及１４５±１１比３０７±１００，Ｐ＜０．０５）。提示ＩＮＳ时，ＰＢＭＣ中ＩＬ－１β及其受体拮抗剂基因表达异常。作者认为其原因可能与多个细胞因子间相互调节失常有关，并分析了所用生物素标记探针杂交技术在儿科临床应用中的优越性。

he pathogenesis of INS is related to the disorderof cellular immunity. Using the in situ hybridization ofbiotin-labeled probe and streptavidin-alkaline phos-phatase conjugate detection sytem. we quantitativelyanalysed by ture colour medical image analysis systemthe expression of IL-1beta , IL-1ra mRNA of peripher-al blood mononuclear cells (PBMC ) from 4 cases of id-iopathic nephrotic syndrome. IL-1beta IL-1ra mRNAexprossion of PBMC in INS were significantly lowerthan those of the normal control (107. 63±20. 80 vs195. 56±31. 14; 144. 45±11. 42 vs 370. 38±100. 48,respectively, P<0. 05 ). The expression of IL-1betamRNA was significantly lower than the IL-1ra mRNAexnression of PBMC in INS (107. 63±20. 80 vs 144.45±11. 42 , P<0. 05 ) . There existed dysregulation ofIL-1 beta, IL-1ra mRNA expression in INS. The ad-vantages of biotin-labelled probe in situ hybridizationwere also discussed.