慢性肾衰淋巴细胞功能缺陷与白介素2／白介素2受体系统之间的关系

THE RELATIONSHIP BETWEEN THE DYS-FUNCTION OF LYMPHOCYTE AND IL-2/IL-2RSYSTEM IN CRF

观察了４０例慢性肾衰（ＣＲＦ）病人淋巴细胞增殖反应与白介素２／白介素２受体（ＩＬ－２／ＩＬ－２Ｒ）系统之间的关系。结果表明：（１）ＣＲＦ病人淋巴细胞在体外培养时，ＩＬ－２产生水平以及对丝裂素原和抗原刺激的增殖反应明显低于正常组，培养上清ＩＬ－２水平与淋巴细胞转化率呈正相关。外源性，ＩＬ－２可明显增强ＣＲＦ及正常组的淋巴细胞增殖反应，但前者的增幅明显高于正常对照。（２）ＣＲＦ组淋巴细胞表面ＩＬ－２Ｒ的自发表达及血浆和淋巴细胞培养上清中ＳＩＬ－２Ｒ水平均较正常明显增高，血浆ｓＩＬ－２Ｒ与血浆肌酐水平呈正相关。在维持性血透病人，血浆ｓＩＬ－２Ｒ并与透析时间有关。由此表明，ＣＲＦ时的淋巴细胞增殖反应缺陷与ＩＬ－２水平低下有关，而后者可能是因淋巴细胞生成减少以及淋巴细胞预激活而致ＩＬ－２吸收增加所致。

he relationship between the function of lympho-cyte and IL-2/IL-2R system in CRF was observed.The results showed: 1. The proliferative response oflymphocytes after stimulation with mitogens or antigenwere significantly reduced and the in vitro productionof IL-2 after such stimulation in parallel cultures wasfound to be lower in the patient group. Exogenous IL-2 augmented the proliferation of lymphocytes fromboth patients and normal subjects, but the magnitudeof proliferation was higher in the patients with CRF. 2.The spontaneous expression of IL-2R on the lympho-cyte membrane in resting states and the release of solu-ble IL-2R by lectinstimulated lymphocytes in culturewere significantly higher in the patient group , as wellas the level of sIL-2R in uremic plasma.These results suggested that the impaired prolifer-ative responsiveness of uremic lymphocytes might berelated to reduced IL-2 activity which may be the re-duced production of IL-2 and the increased absorptionof IL-2 caused by the preactivation of the lymphocytse.