肾病综合征患儿人类白细胞抗原－DRB1基因分型

HLA-DRB1 genotyping in children with nephrotic syndrome

为进一步明确人类白细胞抗原（ＨＬＡ）与肾病综合征的关系，应用聚合酶链反应（ＰＣＲ）／顺序特异的寡核苷酸（ＳＳＯ）探针的方法对２３名上海地区激素敏感型肾病综合征患儿进行了ＨＬＡ－ＤＲＢ１等位基因的频率分析，发现ＨＬＡ－ＤＲＢ１０７与该病相关（Ｐ校正Ｐｃｏｒｒ＝９．０２×１０－３，相对危险度，ＲＲ＝８．１５），ＨＬＡ－ＤＲＢ１０９与该病的频复发相关（Ｐｃｏｒｒ＝２．５１×１０－２，ＲＲ＝２０．７７），用ＰＣＲ／ＳＳＯ分型方法可精确分析与肾病综合征相关的ＨＬＡ等位基因。

o determine the relationship between HLA and steroid sensitive nephrotic syndrome(SSNS),the frequencies of the HLA-DRB1 alleles by polymerase chain reaction (PCR)/ sequence-spe-cific oligonucleotide (SSO) probes in 23 Chinese SsNS children from Shanghai region were investigated. Asignificant association with HLA-DRB1 07 was demonstrated(Pc= 9. 02 ×10-3,RR= 8. 15). HLA-DRB1 09 was associated with the frequent relapses of the disease(Pc= 2.51×10-2,RR=20.77).The use of SSO typing provides an opportunity for refined analysis of disease associated HLA alleles.