戊型肝炎病毒中国株结构区ORF2　cDNA的克隆及表达

Cloning and Expression of the Open Reading Frame 2 cDNA from a Chinese Hepatitis E Virus Strain

将成型肝炎病毒（ＨＥＶ）中国株结构区第二开放读码框架（ＯＲＦ２）８３７ｂｐｃＤＮＡ片段插入ｐＧＥＸ１λＴ质粒中进行表达。经免疫吸印法证明，该质粒表达的融合蛋白具有识别抗－ＨＥＶ的抗原表位，可用于制备抗－ＨＥＶ检测试剂。

A 837 base cDNA fragment from the second open reading frame of the structual region of a Chinese HEV strain was inserted into the pGEX1λT expression plasmid.The Western blot assay indicated that the fusion protein expressed by this plasmid contains epitopes recongnized as anti-HEV antibodies.It demonstrates that this fusion protein is useful for the development of anti-HEV detection systems.