慢性宫颈炎患者宫颈中人乳头瘤病毒基因的检测

Detection of Human Papillomavirus DNA in cervical samples from Patients with Chronic Cervicitis

应用聚合酶链反应技术（ＰＣＲ）检测正常妇女与慢性宫颈炎患者宫颈人乳头瘤病毒（ＨＰＶ）感染情况的结果显示，南京市慢性宫颈炎患者ＨＰＶ－ＤＮＡ总阳性率达５８．６％；ＨＰＶ６，１１，１６，１８型阳性率分别为２９．３％，３０．７％，２８．６％和３４．３％，而正常妇女中ＨＰＶ总阳性率为１９．４％，４个型别ＨＰＶ阳性率为９．７％，１６．１％，３．２％和３．２％，明显低于宫颈炎患者。如皋市慢性宫颈炎患者ＨＰＶ－ＤＮＡ总阳性率高达８１．８％，而４个型别阳性率分别为３４．１％，２７．３％，５０．０％和５９．１％，总阳性率及ＨＰＶ１６，１８型阳性率显著高于南京市患者，这可能与该市较高的宫颈癌发生率有一定关系。

Polymerase chain reaction (PCR) was used to detect human papillomavirus DNA (HPV-DNA) in normal women and women with chronic cervicitis. In the group of patients with chronic cervicitis in Nanyng, HPV-DNA was detected in 58.6%,among them, HPV 6, 11, 16 and 18 being 29.3%, 30.7%, 28.6% and 34.3% respectively. But in normal women, HPV-DNA was only 19.4% in toal and HPV 6, 11, 16, 18 being 9.7%, 16.1%, 3.2% and 3.2% respectively. In the samples obtained from patients with chronic cervicitis in Rugao, the positive rate of HPV-DNA was 81.8%, while that of HPV6,11.16,18 was34.1 %, 27.3%, 50.0%, 59.1 % seperately. The results indicated that HPV infection in normal women was clearly fewer than those with chronic cervicitis. The frequency of HPV infection, especially HPV16 and HPV 18, in women with chronic cervicitis was obviously higher in Rugao than that in Nanbng. It might implicate the high incidence of cervicl cancer in Rugao.