丙型肝炎病毒基因组部分E2／NS1蛋白基因在大肠杆菌中的表达

Expression of Hepatitis C Virus E2/NS1 Gene in E. coli

在大肠杆菌中表达了丙型肝炎病毒基因组的部分Ｅ２／ＮＳ１蛋白（氨基酸３６４－５１２）。表达蛋白经ＳＤＳ－ＰＡＧＥ分析，主要表达带的分子量在～４３０００左右。表达蛋白用于检测已用Ｃ３３ｃ及Ｑ２２检测过的血清，发现Ｅ２ＮＳ１引抗体阳性率占Ｃ３３ｃ及Ｑ２２抗体阳性血清的２０％，尚没有发现单独Ｅ２／ＮＳ１抗体阳性的血清。

Hepatitis C virus E2/NS1 gene was expressed in E.coli using prokaryotic expression vector pGEX3x system. The glutathione S-transferase(GST) gene fusion protein was purified from bacterial lysates by affinity chromatography using prepacked glutathione sepharose CL 4B column. The expression protein was analysed by SDS-PAGE and Western blot, a 43000 band was mainly seen. When tested by enzyme immunoassay (EIA), the E2/NS1 antibody positive rate among C33c and C22 positive sera was 20%. At present no serum was found to be E2/NS1 positive only.