大鼠肝脏隔离灌注不同剂量5－氟脲嘧啶静脉血中前列环素与血栓素A_2的变化

The changes of thromboxane B2 and 6-ke-to-PGF 1α in vein blood during isolated liverperfusion with different doses 5-Fu in rats

应用大鼠肝脏隔离灌注模型，以不同剂量５－氟脲嘧啶（５－Ｆｕ）行门静脉灌注，用放免法检测下腔静脉血中ＴＸＢ＿２与６－ｋｅｔｏ－ＰＧＦ＿（１α）水平，结果表明，ＩＬＰ５－Ｆｕ３００ｍｇ／ｋｇ组术后３天ＴＸＢ＿２明显升高，６－ｋｅｔｏ－ＰＧＦ＿（１α）升高不显著；ＩＬＰ５－Ｆｕ１５０ｍｇ／ｋｇ组与灌注平衡液组ＴＸＢ＿２比术前明显升高，但两组间无明显变化，６－ｋｅｔｏ－ＰＧＦ＿（１α）与术前相比无明显变化。在ＩＬＰ５－Ｆｕ３００ｍｇ／ｋｇ组，ＴＸＢ＿２／６－ｋｅｔｏ－ＰＧＦ＿（１α）值与ＧＰＴ值呈正相关。提示ＴＸＡ＿２与ＰＧＩ＿２平衡的改变与超ＭＴＤ的５－ＦｕＩＬＰ时肝组织损伤密切相关。

This papar presents that thromboxane B2(TXB2) and 6-keto-PGF1α levels in the bloodof inferior vena cava were determined by ra-dioimmunoassay method by means of isolatedliver perfusion(ILP)model with different dos-es 5-Fu through portal vein route in rats.Theresults showed that TXB2 levels were signifi-cantly increased and 6-keto-PGF 1α levels notincreased in group of ILP with 300mg/kg 5-Fuat the third postoperative day.TXB2 levels ingroups of ILP with 150mg/kg 5-Fu and with-out 5-Fu were higher than preoperative levelsand TXB2 levels between these two groupsunchanged.The 6-keto-PGF1α levels have noapparent changes as compared with preopera-tive levels。There was a high positive relativityin TXB2/6-keto-PGF1αratios and SGPT ingroup of ILP with 300mg/kg 5-Fu.The re-sults indicated that the imbalance between lev-els of TXB2 and 6-keto-PGF1α was associatedwith hepatic injuries during ILP with overthan MTD5-Fu.