AM脑内移植后PD鼠纹状体内单胺类神经介质及D_2受体变化的研究

The changes of the levels of monoaminer-gic neurotransmitters and the densities ofdopamine D_2 receptors following intracerebralimplantation of AM grafts

采用高效液相色谱——电化学检测法测定纹状体内单胺类递质多巴胺及其代谢产物３，４一二羟基苯乙酸，去甲肾上腺素含量，利用放射配体结合分析法检测多巴胺Ｄ＿２受体密度及亲和力，以研究肾上腺髓质（ＡＭ）脑内移植后帕金弥补单胺类神经介质缺乏，促使超敏的Ｄ＿２受体下调近至正常水平。结果提示ＡＭ脑内移植后的神经再支配可能是移植物长期发挥作用的机制，ＡＭ脑内移植是治疗ＰＤ的有效手段。

In order to investigate the intrastriatalneurochemical changes of Parkinson’s disease(PD) following intracerebral implantation of a-drenal medulla(AM)and demonstrate themechanism of grafts of AM curing parkinson-ism,the levels of the neurotransmitter of dopamine and its metabolite,3,4-dihydrox- yphenylacetic acid and noradrenaline,werequantitatively measured by HPLC- ECD. Meanwhile,the densities and the affinities ofdopamine D_2 receptors were also detected byusing the method of radioligand binding as-say. It was found the grafts of AM could al-mostly provide a substantial restoration of thedopaminergic neurotransmitters in the previ-ously denervated striatum,make the supersen-sitivity of the densities of dopamine D_2 recep-tors down-regulation closed to normal leveland recover the motor asymmetry of PD rats.Our observations reveal that intracerebraltransplantation of AM grafts is an efficientway for the tneatment of PD .The evidences imply that the renervation between the hostand the grafts are the possible basic mecha-nisms for the improvements.