脑立体定向接种C_6细胞建立大鼠脑胶质瘤模型

Model of Rat Cerebral Glioma established by Stereotactic Inoculation of C6 Cells

应用脑立体定向术将Ｃ＿６．细胞接种在Ｓｐｒｅｇｕｅ－Ｄａｗｌｅｙ大鼠脑尾状核，同时留置永久性聚乙烯导管建立的大鼠脑胶质瘤模型方法。将２０只ＳＤ大鼠按肿瘤生长时间分为４组。分别在接种后１、２、３、４周时进行ＭＲＩ检查，病理解剖，病理组织学以及ＧＦＡＰ和Ｓ－１００免疫组化检查。结果表明该方法建立胶质瘤模型不仅较为接近人脑胶质瘤生长特性，而且颅内生长稳定，无颅外种植生长，适于胶质瘤实验治疗研究需要。

The model of rat cerebral glioma estab-lished by stereotactic inoculating C6 cells intoSpregue-Dawley rat caudate nucleus,followingpermanant implantation of a polyvinylcatheter ,is discribed in this paper,MRI,pathoanatomy,histology and both GFAP andS一100 protein immunohischemistry of gliomamodels were respectivily observed in 20 rats,which were divided into four groups at lst,2nd, 3rd and 4th week of postinoculation,Theexperimental results demonstrated that tumorof models were not only similar to humanglioma growing characters, but also stablegrowing at intracerebral and no extracranialgrowth.It is can be better used in experimen-tal therapy of gliomas.