肿瘤坏死因子在梗阻性黄疸鼠肝中的免疫组化分析

IMMUNOHISTOCHEMICAL ANALYSIS OF TNF IN THE LIVER IN OBSTRUCTIVE JAUNICE RATS.

通过利用组织免疫组化技术，研究ＴＮＦ在梗阻性黄疸中肝脏中的表达。结果表明ＴＮＦ主要分布于梗黄肝组织的ＫＣ及ＰＭＮ细胞中，ＢＤＬ组中有较少的表达，给予内毒素刺激后ＴＮＦ产生伴随组织病理改变的加重相应增多，ＫＣ及ＰＭＮ是ＴＮＦ产生的主要来源，是胆道术后并发症发生的部分原因之一，抑制其产生将有益于减少黄疸术后并发症的发生。

The aim of the present study is at assessthe expression of tumor necrosis factor(TNF)in liver in the obstructive jaundice by ABC im-munohistochemical analysis. The result Showsthat presence of staining for TNF were foundin BDL animals,but not in the control. Afterlipopolysaccharide(LPS)0.7mg/kg intraperi-toneally seven days post BDL. Quantitation ofstaining cells were significantly higer accom- panied morphological change further increase. The positive cells were consistent with kupffercells(KC)and polymorphonuclear leukocytes(PMN). Increase of TNF amy contribute toliver cells necrosis post obstructive jaundicewith infection and cause the complications af- ter biliarical operation. It would be beneficalfor decrease the complication after surgerythrough block the production of TNF.