大块肝切除后急性肝损伤治疗的研究

The studies on treatment of acute hepaticinjury after large partial hepatectomy

将６０只大鼠随机分为２组，一组腹腔内注射肝细胞生长因子（ＨＧＦ）和１，６二磷酸果糖（ＦＤＰ），另一组代之以生理盐水做为对照，分别于术后３、７、１４天检测血清酶学变化、胃泌素含量和体外肝细胞培养蛋白质合成及ＤＮＡ合成。结果发现：治疗组大鼠术后３天ＡＬＴ较对照组迅速降低（Ｐ＜０．０１）；血浆脯肽酶（ＰＬＤ）在术后７天、１４天低于对照组（Ｐ＜０．０５）；血清胃泌素测定在术后３天、７天治疗组高于对照组（Ｐ＜０．０１）；肝细胞体外原代培养￣３Ｈ－亮氨酸掺入法显示治疗组术后３天蛋白质合成明显高于对照组（Ｐ＜０．０１）；￣３Ｈ－ＴｄＲ掺入肝细胞ＤＮＡ合成，治疗组术后各期均非常显著高于对照组（Ｐ＜０．０１）。结果证实大鼠大块肝切除后应用ＨＧＦ和ＦＤＰ，对急性肝损伤有重要的治疗作用。

Sixty tats with acute hepatic injury due tolarge partial hepatectomy(70%)were dividedinto two groups at random. Group 1 was treat-ed by HGF and FDP through peritoneal cavityinfusion and the other group receiving physio-logic saline served as a control group. Thechanges in the serum enzymes , gastrin andDNA synthesis protein synthesis of hepato-cytes were evaluated. Results demonstrated in-tracellular DNA and protein were significantlyhigher in the treated group than those in thecontrol group(P<0. 01). In contrast to con-trol group ALT in treated group decreasedrapidly at 3rd day after large partial hepatec-tomy(P< 0.01).PLD in control group in-creased significantly at 7th and 14th day afteroperation comparied with treatment group(P<0.05). Serum gastrin in treated group wasevaluated at 3th and 7th day and comparedwith control group(P<0.01).The resultssuggest that postoperative intervention ofHGF and FDP in rats could improve the liverregeneration ,depresse the activities of ALTand PLD,preserve the function of hepatocytesand accelerate recovery of hepatocytes. It isconcluded that HGF and FDP play importantroles in the treatment of acute hepatic injury.