血小板活化因子拮抗剂对大鼠烫伤早期肠道细菌移位的影响

The effect of platelet-activating factor antagonists onearly bacteria translocation of rat after burn injury

实验采用３０％ＩＩＩ度Ｗｉｓｔａｒ大鼠烫伤模型，随机分为正常对照、烫伤和血小板活化因子（ＰＡＦ）拮抗剂治疗三组。动物灌注吖啶橙标记的大肠杆菌，于烫伤后不同时相活杀，取肠系膜淋巴结、肝、肺组织进行细菌定量培养及菌种鉴定，并用荧光显微镜观察组织匀浆液的细菌数。结果显示，应用ＰＡＦ拮抗剂ＷＢＥ２１７０治疗组大鼠肠系膜淋巴结、肝、肺组织细菌量明显减少，与非治疗组比较差异显著（Ｐ＜０．００１）。烫伤组肠系膜淋巴结、肝、肺组织荧光菌检出率分别为１００％、８０．０％及５０．０％；冶疗组分别为４０．０％、３０．０％及２０．０％。结果表明ＷＢＥ２１７０对大鼠烫伤后肠源性感染具有一定防治作用。

Abstract30%third degree burn model of Wistar rat wasused in this experiment. The animals were dividedrandomly into three groups (normal control,burn ,andplatelet-activating factor antagonist treatment).Afterpoured E. coli which labelled with acridine orange intointestine, the rats were killed at 6,12, 24,and 48hrspostburn,the bacteria in mesentery lymph node(MLN),liver and pulmonary orgnisms were culturedand counted , also observed by fluorescent microscopydirectly.The results showed that,in PAF antagonist(WEB2170 ) treatment group, the quantity of bacteriain MLN, liver and lung were decreased significantly (P<0.001).The labelled bacteria in MLN,liver andlung of burn group were 100%、80.0%,and 50.0%respectively compared with 40.0%, 30,0%, and20. 0%in treatment group. It is suggested that WEB2170 could protect the intestine from bacteria translo-cation after burn injury.