摘要
实验采用30%III度Wistar大鼠烫伤模型,随机分为正常对照、烫伤和血小板活化因子(PAF)拮抗剂治疗三组。动物灌注吖啶橙标记的大肠杆菌,于烫伤后不同时相活杀,取肠系膜淋巴结、肝、肺组织进行细菌定量培养及菌种鉴定,并用荧光显微镜观察组织匀浆液的细菌数。结果显示,应用PAF拮抗剂WBE2170治疗组大鼠肠系膜淋巴结、肝、肺组织细菌量明显减少,与非治疗组比较差异显著(P<0.001)。烫伤组肠系膜淋巴结、肝、肺组织荧光菌检出率分别为100%、80.0%及50.0%;冶疗组分别为40.0%、30.0%及20.0%。结果表明WBE2170对大鼠烫伤后肠源性感染具有一定防治作用。
Abstract30%third degree burn model of Wistar rat wasused in this experiment. The animals were dividedrandomly into three groups (normal control,burn ,andplatelet-activating factor antagonist treatment).Afterpoured E. coli which labelled with acridine orange intointestine, the rats were killed at 6,12, 24,and 48hrspostburn,the bacteria in mesentery lymph node(MLN),liver and pulmonary orgnisms were culturedand counted , also observed by fluorescent microscopydirectly.The results showed that,in PAF antagonist(WEB2170 ) treatment group, the quantity of bacteriain MLN, liver and lung were decreased significantly (P<0.001).The labelled bacteria in MLN,liver andlung of burn group were 100%、80.0%,and 50.0%respectively compared with 40.0%, 30,0%, and20. 0%in treatment group. It is suggested that WEB2170 could protect the intestine from bacteria translo-cation after burn injury.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1995年第4期217-218,共2页
Chinese Journal of Surgery
基金
国家自然科学基金