呼吸道合胞病毒G基因的扩增

AMPLIFICATION OF G GENE OF RESPIRATORY SYNCYTIAL VIRUS IN CHINA

用未纯化的呼吸道合胞病毒(RSV)细胞混和物,采用改良胍—热酚法提取病毒mRNA,反转录合成cDNA,经PCR扩增得到大小约920bp的产物。经PstⅠ酶切表明其中含有PstⅠ酶切位点,证实其产物确为RSV G基因。此法简便、特异、敏感、快速。由于RSV极不稳定,RNA甚易降解,此法值得推广应用。

From cells infected by respiratory syncytial virus (RSV),cDNA was synthesized through reverse transcription by using improved method of guanidine and heated phenol. An about 920bp product was obtained by PCR. It indicated that the product was RSV G gene because it had Pst I digestion site.