人宫颈癌中C-myc、H-ras和HPV基因的检测和分析

C-myc AND H-ras CELLULAR ONCOGENES AND HUMAN PAPILLOMAVIRUS IN CERVICAL CARCINOMA

收集60例宫颈癌活检组织,对同一病例同时进行HPV、C—myc、H—ras对比研究。采用HPV高保守序列区一对共有引物检测多型HPV基因型的存在,发现85％(51/60)的组织中存在HPV,经限制性片段长度多态性分析,HPV16占78.43％(40/51),HPV18占21.65％(11/51)。此外,用Southern印迹法对60例宫颈癌组织中C—myc、H—ras癌基因进行分析,地高辛标记的C—myc、H—ras探针进行杂交,发现C—myc扩增占33％(19/60),既有C—myc扩增又有重排者占8.3％(5/60),H—ras扩增占6.6％(4/60),C—myc扩增明显高于H—ras(33％与6.6％)。实验表明,C—myc扩增在HPV感染阳性组织中占89.47％(17/19),而HPV16占76.5％(13/17),HPV18仅占17.64％(3/17)。提示,高危HPV在致癌过程中发挥着重要作用,而C—myc参与了HPV16和HPV18的致癌过程,由于HPV DNA整合到细胞基因组内,则引起对细胞原癌基因的扩增或被激活。

To analyze the role of human papillomavirus (HPV) infection and C-myc and H-ras oncogene activation in development of cervical carcinoma,60 biopsies from cervical carcinoma patients were examined. HPV DNA was detected by consensus polymerase chain reaction. Tn addition ,the same DNAs have been analysed for amplifications the C-myc,H-ras gene loci by Southern blotting. The results showed a HPV positive rate of 85% (51/60) in 60 cervical carcinoma biopsy tissues. HPV 16 sequences were detected in a total of 51 (78. 43%) and HPV 18 sequences in 11 of the 51 (21. 56%). Amplification of C-myc oncogene was found in 19/60 cancer,of which 13 were HPV 16 positive,and three also positive for HPV 18. The amplification of ras oncogene was detected in 4/60 specimens. The amplification of myc was more frequently amplified than that of ras. It suggests that C-myc plays a role in the carcinogenesis of HPV 1 6 and HPV 18. Therefore,it is most likely that the activation of protooncogenes may result in the insertion of HPV 16 DNA into cellular genomes.