IL-3基因转染的肿瘤细胞体内对巨噬细胞数目和功能的影响

EFFECTS OF IL-3 GENE-TRANSFECTED TUMOR CELLS ON THE NUMBER AND FUNCTIONS OF PERITONEAL M ACROPHAGES IN VIVO

曾发现转染IL—3基因的黑色素瘤细胞(B16—IL—3)体内致瘤性下降,肿瘤组织有包括巨噬细胞在内的大量炎症细胞浸润。进而研究了C57BL/6小鼠在皮下接种B16—IL—3细胞后,腹腔巨噬细胞数目和功能的变化。在接种B16-IL-3细胞后第4天,腹腔巨噬细胞的数目就升高1倍,接种后第10～15天升高5～6倍。而且体外无需LPS的刺激,腹腔巨噬细胞就能分泌一定水平的细胞因子IL—1、IL—6和TNF,具有较强的杀伤活性,体外经LPS刺激后,其分泌水平和杀伤活性继续升高,明显高于对照组。此外。腹腔巨噬细胞MHC二类抗原Ia的表达也明显增强。提示B16—IL—3细胞分泌的IL—3有效地激活了体内的巨噬细胞,这可能是B16—IL—3细胞体内致瘤性下降的原因之一。

The author had found that the tumorigenicity of B I 6 melanoma cells transfected with 1L-3 gene decreased ,with a large amount of inflammatory cells infiltrating in the tumor tissues. In this study,the effects of IL-3 in riro secretion on the peritoneal macrophages were investigated. The numbers of peritoneal macrophages doubled 4 days after inoculation of B16-IL 3 cells, in creased by 4-5 times after 10-15 days. The freshly prepared marophages from BI6-IL-3 inoculated mice could secret some IL-l.IL-6 and TNF.and exert high tumoricidal activity. The cytokine secretion and cytotoxicity were greatly enhanced after induction of LPS in ritro. Moreover,their la antigen expression was significantly improved. These data show that IL-3 secreted by B16-IL-3 cells in nivo has effectively activated the peritoneal macrophages, to some extent, which may account for the decreased tumorigenicity of the IL-3 gene transfected melanoma cells.