活动力不足精子的线粒体变化──形态学和计量学研究

Changes of Micochonaria in hypoactive Spermtozoa──Morphological and Stereological Study

通过电镜观察和体视学方法，研究活动力不足精子的线粒体变化．结果是运动力不足的精子尾部中段存在较多胞质，线粒体排列方式呈现异常，线粒体内部的结构有退化现象。运动力不足的精子线粒体的体密度（ＶＶ）、外膜面密度（ＳＶ）和面数密度（ＮＡ）均小于对照组（Ｐ＜０．０５），线粒体的外膜比表面（ＲＳＶ）则大于对照组（Ｐ＜０．０１），而线粒体的平均体积及平均外表面积和对照组比无差异（Ｐ＞０．５）．结果提示线粒体在形态学和计量学方面发生了改变，这可能是导致精子运动力低下的原因之一．

By transmission electron microscopy and stereological techniques, the mitochondria of hypoactive spermtozoa were studied.The results showed:there was more cytoplasm in the middle part of the tail asthenospermia spermtozoa; the arrangment of mitochondria was abnormal; atrophied phenomena were noted in internal structure of the mitochondria; the volume density(VV),surface density(SV) of outer membrance and numerical density on area (NA)of mitochondria in the asthenospermia were smaller than those in the control(P<0.05);the ratio of surface to volume for the outer membrance phase(RSV)of mitochondia of asthenospermia was larger than that in the control(P<0.01); there was no significant difference between the mean volume and mean surface of mitochondria of asthenospermia and that of the control(P>0.5).The results suggested that the morphological and sterdlogical changes of the mitochonaria of the spermtozoa may be one of reasons effecting spermatozoal motility.