摘要
根据tal-1基因缺失上下游序列设计一对引物,应用多聚酶链反应(PCR)技术对10株人白血病细胞株和70例小儿急性白血病骨髓标本进行tal-1基因缺失分析。结果CEM、HBS-2和RPMI-8402三株T细胞白血病(T-ALL)细胞株以及3/16例T-ALL发现tal-1基因缺失,54例其它类型急性白血病均未见基因缺失,而且具tal-1基因缺失的T-ALL细胞均为胸腺发育I期,免疫表型特征为CD_2+、CD_7+、CD_1 ̄-、CD_4 ̄-和CD_8 ̄-。PCR方法敏感性达10 ̄(-4)。说明tal-1缺失发生于部分T-ALL中,可用于微小残留病的检测。
wo primers were designed according to up-anddown stream sequences of the deletion site of tal.1gene. TaL-1 gene deletion was assayed by PCR methodhten human leukemia cell lines and 70 children withacute leukemia.The deletions were detected in three Tcell eukemia cell lines (CEM, HBS-2, RPMI 8402 )and 3 of 16 T-ALL patients, The leukemic cells ofthree patients with tal-1 gene deletion were at stage Iof thymus differentiation with a immunophenotype ofCD_2 ̄ +,CD_7 ̄+,CD_1 ̄-,CD_4 ̄-,CD_8 ̄-,The sensitivity of PCR was 10 ̄(-4). It suggested that tal-1 gene deletionoccured in some of T-ALL patient and migth be usefulindetection of MRD.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第8期417-418,共2页
Chinese Journal of Hematology
基金
国家自然科学基金