小儿急性T淋巴细胞白血病tal－1基因缺失分析

ANALYSIS OF TAL-1 GENE DELETION IN CHILDHOOD ACUTE T CELL LEUKEMIA

根据ｔａｌ－１基因缺失上下游序列设计一对引物，应用多聚酶链反应（ＰＣＲ）技术对１０株人白血病细胞株和７０例小儿急性白血病骨髓标本进行ｔａｌ－１基因缺失分析。结果ＣＥＭ、ＨＢＳ－２和ＲＰＭＩ－８４０２三株Ｔ细胞白血病（Ｔ－ＡＬＬ）细胞株以及３／１６例Ｔ－ＡＬＬ发现ｔａｌ－１基因缺失，５４例其它类型急性白血病均未见基因缺失，而且具ｔａｌ－１基因缺失的Ｔ－ＡＬＬ细胞均为胸腺发育Ｉ期，免疫表型特征为ＣＤ＿２＋、ＣＤ＿７＋、ＣＤ＿１￣－、ＣＤ＿４￣－和ＣＤ＿８￣－。ＰＣＲ方法敏感性达１０￣（－４）。说明ｔａｌ－１缺失发生于部分Ｔ－ＡＬＬ中，可用于微小残留病的检测。

wo primers were designed according to up-anddown stream sequences of the deletion site of tal.1gene. TaL-1 gene deletion was assayed by PCR methodhten human leukemia cell lines and 70 children withacute leukemia.The deletions were detected in three Tcell eukemia cell lines (CEM, HBS-2, RPMI 8402 )and 3 of 16 T-ALL patients, The leukemic cells ofthree patients with tal-1 gene deletion were at stage Iof thymus differentiation with a immunophenotype ofCD_2￣ +,CD_7￣+,CD_1￣-,CD_4￣-,CD_8￣-,The sensitivity of PCR was 10￣(-4). It suggested that tal-1 gene deletionoccured in some of T-ALL patient and migth be usefulindetection of MRD.