葡萄糖－6－磷酸脱氢酶缺乏红细胞脂质过氧化的研究

STUDY ON THE LIPOPEROXIDATION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE OEFICIENT ERYTHROCYTES

按硫代巴比妥酸法测定了葡萄糖－６－磷酸脱氢酶（Ｇ６ＰＤ）缺乏和正常红细胞的丙二醛（ＭＤＡ）含量，按改良Ｎｉｓｓｅｌｂａｕｍ法测定还原型辅酶ＩＩ（ＮＡＤＰＨ）的含量。Ｇ６ＰＤ缺乏红细胞的ＭＤＡ和ＮＡＤＰＨ分别为３８．７４ｎｍｏｌ／ｇＨｂ和１８０３．６μｍｏｌ／ｇＨｂ；正常红细胞中含量则分别为２５．６０ｎｍｏｌ／ｇＨｂ和２２４２．８ｔＬｍｏｌ／ｇＨｂ。在受到过氧化刺激后，Ｇ６ＰＤ缺乏红细胞的ＭＤＡ生成明显大于正常红细胞，ＮＡＤＰＨ明显减少，而正常红细胞的ＮＡＤＰＨ则增加。维生素Ｅ能保护Ｇ６ＰＤ缺乏红细胞，使其在过氧化刺激下ＭＤＡ生成不增加。研究提示，Ｇ６ＰＤ缺乏红细胞的脂质过氧化损伤比正常严重，其机制是ＮＡＤＰＨ生成不足。

he malonyldialdehyde(MDA)and NADPH levels were detected in G6PD deficient and normal erythro-cytes.The MDA and NADPH levels were 38.74 nmol/gHb and 1803.6μmol/gHb in G6PD deficient erythrocytes and 25.6nmol/gHb and 2242.8μmol/gHb in normal erythrocytes,respectively. Under oxidative stress,the G6PD deficient erythrocytes generated more MDA than normal.and the NADPH level was increased in normal erythrocytes but decreased inG6PD deficient erythrocytes,Vitamin E could protectG6PD deficient erythrocytes from oxidative damage,the production of MDA did not increase under oxida-tive stress if tbe G6PD deficient ervthrocvtes weretreated with vitamin E .The results indicated that the degree of lipoperoxidation was more severe in G6PDdeficient erythrocytes than in normal ones.The mech-anism of the overlipoperoxidation in G6PD deficienterythrocytes is the insufficient production of NADPH.