流式细胞术分析脑益嗪对成纤维细胞的调控作用

THE MODULAT0RY EFFECTS OF CINNARIZINE ON FIBROBLASTS WERE ANALYSED BY FLOW CYTOMETRY

用流式细胞术测定不同剂量的脑益嗪（ＣＩＮ）对培养中３Ｔ６成纤维细胞的影响，包括检测细胞生长周期、ＤＮＡ含量、分裂增值指数及Ｇ１期和Ｇ２期蛋白质含量的变化。发现用脑益嗪后Ｇ１期细胞数增加，而Ｓ期细胞数减少，但无明显的量效关系；ＤＮＡ含量及分裂增殖指数下降；Ｇ１期及Ｇ２期蛋白质含量明显高于对照组。结果表明，脑益嗪可阻滞Ｇ１→Ｓ进程，使细胞堆积于Ｇ１期，减少ＤＮＡ含量及细胞分裂，增加蛋白质含量，使３Ｔ６细胞呈现不平衡生长状态。

ffects of different doses of Cinnarizine(CIN)on cultured 3T6 fibroblasts were analysedby flow cytometry,as well as assayed cell growth cycles,DNA contents,Proliferationindices(PI),G1 and G2 phases protein contents.After CIN was used,the number of G1 phasecells was increased,that of S phase decreased,but thay had no significant positive dose andeffect relation. Although DNA contents and PI decreased,protein contents of G1 and G2phases increased.The results showed that CIN may delay G1→S,and make cells expressunblanced growth.