结核杆菌－DNA和肿瘤相关抗原对良、恶性腹水的鉴别价值

DIAGNOSTIC VALUE OF TB-DNA、TAA IN BENIGN AND MALIGNANT ASCITES

我们应用放射免疫法（ＲＩＡ）和聚合酶链反应（ＰＣＲ）法对１０６例良、恶性腹水（良性组３５例、结核组３５例、恶性组３６例）进行了结核杆菌ＤＮＡ（ＴＢ－ＤＮＡ）和肿瘤相关抗原（ＴＡＡ）的癌胚抗原（ＣＥＡ）、糖链抗原（ＣＡ１９－９、ＣＡ５０）的测定。结果：（１）ＴＡＡ三项指标：恶性组显著高于良性组和结核组（Ｐ＜０．０１）。单项ＣＥＡ、ＣＡ１９－９和ＣＡ５０对恶性腹水的敏感性分别为５８％、７０％、６９％，特异性均为１００％；联合三项检测诊断恶性腹水的敏感性为９６％，特异性为１００％；（２）ＴＢ－ＤＮＡ：对结核性腹水的敏感性、特异性分别为９７．１４％、９５．７５％

sing radio-immunoassay(RIA)、 polymerase chain reaction(PCR),We had determinedtuberculous deoxyribonucleic acid(TB-DNA)、 the tumor-associated antigen(TAA), such as CEA,CA19-9、 CA50 in 106 benign and malignant ascites(benign 35、tuberculous 35 and malignant 36).Results:(1) The three indexes of TAA in the malignant group were significantly higher than thosein the benign and the tuberculous group(P<0.01).The sensitivity of single item CEA、 CA 19-9 orCA50 was 58%、70%、69%respectively,the specificity of all three being l00%,Sensitivity of thethree markers combined in diagnosing malignant ascites was 96%,and the specificity l00%;(2)TB-DNA: The sensitivity and specificity to tubeculous ascites were 97.14%and 95.75 % respectively.