Wistar鼠单侧隐睾对侧已降睾丸的病理改变

The Pathological Changes of the Contralateral Descended Testis in Wistar Mice With Unilateral Undescended Testis

将Ｗｉｓｔａｒ雄性日龄１４天幼鼠复制单侧隐睾（右侧）模型，在日龄２８，５０和１００天时处死，取两侧睾丸。以日龄配对的未手术组和模拟组作对照。结果日龄２８天和５０天时隐睾组对侧已降睾丸（左侧，ｃｏｎｔｒａｌａｔｅｒａｌｄｅｓｃｅｎｄｅｄｔｅｓｔｉｓ，ＣＤＴ）的重量、容积、平均精细管直径（ｍｅａｎａｅｍｉｎｉｆｅｒｏｕｓｔｕｂｕｌｅｄｉａｍｅｔｒｅ，ＭＳＴＤ）和睾丸活检评分（ｔｅｓｔｉｃｕｌａｒｂｉｏｐｓｙｓｃｏｒｅ，ＴＢＳ）与两个对照组左侧睾丸差异无显著意义（Ｐ＞０．０５）。日龄１００天时ＣＤＴ的重量和容积与两个对照组左侧睾丸差异无显著意义（Ｐ＞０．０５）；ＣＤＴ的ＭＳＴＤ２５３．５±３６．１μｍ，ＴＢＳ３．６３±０．５２分；未手术组和模拟组左侧睾丸的ＭＳＴＤ分别为３０３．３±１７．７μｍ和３０３．０±１６．６μｍ，ＴＢＳ９．５８±０．２４分和９．６±０．１４分，ＣＤＴ的ＭＳＴＤ和ＴＢＳ与两个对照组差异有显著意义（Ｐ＜０．０１）。流式细胞计数生殖细胞ＤＮＡ分析，日龄２８天时ＣＤＴ的两倍体细胞群（ｄｉｐｌｏｉｄ，２Ｃ）３０．０８％±９．９７％；未手术组左侧睾丸２Ｃ７５．５１％±１．７６％，两组差异有显著意义（Ｐ?

Gubernaculum of right testis was divided in 36 Wistar mice at 14 days age. Bilateral orchidectomy was performed on 28 days, 50 days and 100 days of age respectively. The changes in weight, volume, mean seminiferous tubular diameter (MSTD), tubular biopsy score(TBS)and DNA of germinal cells of contralateral descended testis (CDT) were observed. No significant change was found in weight, volume.MSTD and TBS of CDT at 28 and 50 days age (P>0.05). At 100 days age, there was still no change in weight and volume of CDT(P>0.05). But there was remarkable change in MSTD and TBS(P<0.01). In flow cytometry, a remarkable reduction of uniploid at 28 days age (P<0. 01) ,and reduction of diploids at 50 days age (P<0. 02) and 100 days age (P<0.01). It is concluded that there are definite pathological changes of contralateral descended testis in Wistar mouse with unilateral undescendant testis.