先天性巨结肠一氧化氮的组织化学观察

Preliminary Observations on Nitric Oxide Synthase Histochemistry of Hirschsprung Disease

采用ＮＡＤＰＨ二磷酸酶组织化学方法，研究先天性巨结肠症ＮＡＮＣ抑制性神经系统。结果提示狭窄段肠管缺乏ＮＯＳ阳性神经丛，肌层内有散在神经纤维，有时可见到粗纤维或小神经干；正常结肠和巨结肠正常段富含阳性神经节细胞和神经纤维。提示ＮＯ与先天性巨结肠发病机理有关。

The nitric oxide synthase(NOS)distribution in the enteric nervous system(NANC inhibition system)was studied by NADPH-diphorase histochemistry method.It revealed completely lack of NOS activity in the neuroplexus and in musculature of aganglionic segment,on the other hand in the ganglionic segment,NOS was abundant in pattern similar to normal colon.It suggests that NO has been involved in the pathophysiology of Hirschsprung disease.