RNA－RNA原位杂交检测心肌中的肠道病毒RNA

Detection of enterovirus RNA in the myocardium by RNA-RNA in situ hybridization

报道将ｐＣＶＢ３－Ｒ１进行体外转录合成柯萨奇Ｂ３病毒负股ＲＮＡ，以同位素３５Ｓ标记作为阳性探针，将ｐＳＰＴ１８的体外转录产物以同位素３５Ｓ标记作为阴性探针，分别对多种经石蜡包埋的心肌切片进行原位杂交，检测肠道病毒ＲＮＡ。阳性探针检测结果显示：急性小鼠柯萨奇Ｂ３病毒性心肌炎心肌均有阳性信号出现；３例急性心肌炎和２例扩张型心肌病心肌各有１例阳性发现；６例风心病病人心脏瓣膜未见阳性信号；正常人和小鼠心肌未见阳性信号。阴性探针检测未发现任何阳性信号。可见此方法特异性高，可对肠道病毒ＲＮＡ进行特异检测。本结果也提示在我国急性心肌炎和扩张型心肌病病人心肌中存在肠道病毒的持续感染。

Abstract Using 35 S-labelled negative-strand RNA probe synthesized in vitro from cloned Coxsackie virus B_3cDNA as a diagnostic probe and that from pSPT18 as a control probe, enterovirus RNA in paraffin-embedded samples of the myocardium from patients was detected by RNA-RNA in situ hybridization. Positive signals were found in the myocardia of all mice with acute Coxsackie virus B_3 myocarditis,one of 3 patients with acute myocarditis and one of 2 patients with dilated cardiomyopathy by the diagnostic probe; while no positive signals by the control probe. The results showed that this inethod is highly specific and persistent infection of enterovirus in the myocardia of patients with myocarditis and dilated cardiomyopathy is present in our country.