摘要
报道将pCVB3-R1进行体外转录合成柯萨奇B3病毒负股RNA,以同位素35S标记作为阳性探针,将pSPT18的体外转录产物以同位素35S标记作为阴性探针,分别对多种经石蜡包埋的心肌切片进行原位杂交,检测肠道病毒RNA。阳性探针检测结果显示:急性小鼠柯萨奇B3病毒性心肌炎心肌均有阳性信号出现;3例急性心肌炎和2例扩张型心肌病心肌各有1例阳性发现;6例风心病病人心脏瓣膜未见阳性信号;正常人和小鼠心肌未见阳性信号。阴性探针检测未发现任何阳性信号。可见此方法特异性高,可对肠道病毒RNA进行特异检测。本结果也提示在我国急性心肌炎和扩张型心肌病病人心肌中存在肠道病毒的持续感染。
Abstract Using 35 S-labelled negative-strand RNA probe synthesized in vitro from cloned Coxsackie virus B_3cDNA as a diagnostic probe and that from pSPT18 as a control probe, enterovirus RNA in paraffin-embedded samples of the myocardium from patients was detected by RNA-RNA in situ hybridization. Positive signals were found in the myocardia of all mice with acute Coxsackie virus B_3 myocarditis,one of 3 patients with acute myocarditis and one of 2 patients with dilated cardiomyopathy by the diagnostic probe; while no positive signals by the control probe. The results showed that this inethod is highly specific and persistent infection of enterovirus in the myocardia of patients with myocarditis and dilated cardiomyopathy is present in our country.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
1995年第2期111-112,共2页
Chinese Journal of Cardiology
基金
上海市科委资助项目