窦房结主、间质形态学改变与年龄的相关研究

Computer microimage analysis of age-related chauges in human sinoatrial node

对１０６例无明显心脏疾患的法医解剖人体的心脏传导系统窦房结（ＳＡＮ）的主、间质形态学进行研究，应用计算机显微图像分析系统测量每例ＳＡＮ纵切最大面积，用网格测微器半定量估算正常人ＳＡＮ内间质所占百分比，发现ＳＡＮ主、间质形态学改变与年龄之间呈现增龄性生理变化，应避免与ＳＡＮ病理改变相混淆。

Abstract The age-related changes in areas and amount of interstitial tissue of human sinoatrial node (SAN) were studied.106 autopsy hearts were taken from cases died at different ages (2 days-83 years) without clinical or pathologic evidence of cardiac diseases.Serial sections 2μm in thickness were prepared parallel to the long axis of SAN.The thin sections selected from the largest ar eas of SAN were stained with HE and Betty's method.The largest areas of sections of SAN in 7 age groups were calculated by computer image analysis system.The area of total SAN and SAN cells increased with age before 20 years of age.In adults,the area reached the maximal plateau.Over 40 years of age,the area of SAN decreased with aging.And the percentage of areas of SAN interstitial tissue were estimated by mesh micrometer.Before 10 years of age,the SAN contains very little interstitium.The percentage of area of SAN interstitium increased with aging (I to V degrees).Age-related changes of the human SAN were discussed.