冷浸泡法保存心脏的实验研究（保存心脏2～6小时）

Experimental Study of Heart Preservation──Heart Preservatiou for 2￣6 Hours with Cold Storage

冷浸泡法保存成年猪心２．５０～６．０８ｈ，利用体外循环装置使１１个心脏离体复苏并测试心功能。在心脏复苏前用温血心停搏液灌注以减轻再灌注损伤。结果表明在灌注温血心停搏液时，心脏无电机械活动，ＭＶＯ２为１．５０±０．９３％。１１个离体心脏经灌注氧合血后７个自动复跳，４个需电击除颤。测试心输出量为１５００～４２５０ｍｌ／ｍｉｎ，平均２７２５±８７５ｍｌ／ｍｉｎ；其中１个心脏保存６．０８ｈ，心输出量为４２００ｍｌ／ｍｉｎ。２个保存心脏的组织学表明除心肌纤维及其间隙轻度水肿外，无不可逆病损。单纯冷浸泡法保存心脏其安全时限一般为４ｈ。本实验采用温血心停搏液灌注法，延长保存期至６ｈ，心肌组织学损害轻。复苏后心功能满意。

In order to explore large animal heart preservation, 13 adult porcine hearts which are similiar to human's in size were used in this experiment.After being infused with cardioplegic solution, the hearts were stored at 4℃ for 2. 5￣6. 08 h with the average of 4. 68 h. Two hearts were studied histologically and the other 11 were resuscitated by a heart-lung machine and warm blood cardioplegia was infused to reduce reperfusion injury. During this time all hearts showed no electric or mechanical activities.while the mean MVO2 was 1. 5 ± 0. 93 ml%. After resuming oxygenated blood perfusion,7 hearts resuscitated spontaneously and 4 required defibrillation. Cardiac output was 1500 ￣ 4250ml/min with an average of 2725 ± 875ml/min. One heart preserved for 6.08 h had a CO of 4200ml/min. Myocardial histology showed that there was no irreversible damage except a slight interstitial and myofibrillar edema.