白血病细胞分泌肿瘤坏死因子α及受诱导分化剂影响

TUMOR NECROSIS FACTOR ALPHA SECRETION INLEUKEMIC CELL AND AFFECTED BY DIFFERENTIATIONINDUCERS

作者观察２８例急性白血病患者原代白血病细胞和ＨＬ－６０细胞自发分泌及在脂多糖（ＬＰＳ）诱导下分泌肿瘤坏死因子α（ＴＮＦα）情况，并用咐醇酯（ＴＰＡ）等５种诱导分化剂诱导原代白血病细胞和ＨＬ－６０细胞，探讨白血病细胞诱导分化与ＴＮＦα分泌之间关系。结果：６例急性单核细胞白血病患者的白血病细胞在ＬＰＳ诱生下可以分泌ＴＮＦα，其中３例在无ＬＰＳ时还可自发分泌ＴＮＦα。用ＴＰＡ等诱导分化后部分原代白血病细胞和ＨＬ－６０细胞可分泌ＴＮＦα，这种分泌与细胞分化有关。

he spontaneous secretion of tumor necrosis factoralpha (TNFα) and induction secretion by lipopolysac-charide (LPS) in HL-60 cells and fresh leukeinic cellsfrom 28 patients with acute leukemia were assayed. Therelation between leukemic cell differentiation and TNFαsecretion in fresh leukemic cells and HL-60 cells after in-duction differentiation bv TPA and 4 other inducers wasstudied. Leukemic cells (monoblast and promonocyte)from 6 patients with acute monocytic leukernia (AMoL)under LPS stimulation could release TNFα,spontaneoussecretion was also detected in 3 out of these 6 AMoLcases . HL-60 cells and leukemic cells from 4 otherAMoL patients , 18 acute lympheeytic leukernia and acute granulocytic leukemia patients did not secrete TNFαbv LPS stirnulation. Fresh leukernic cells and HL-60cells after TPA induction differentiation released TNFαand the secretion of TNFα related to cells differentia-tion.