摘要
建立了一种高度敏感、特异性强并能定量的逆转录／多聚酶链反应（ＲＴ／ＰＣＲ）检测ｍｄｒｌｍＲＮＡ的方法，用该法研究Ｋ５６２／Ｓ及Ｋ５６２／ＶＣＲ细胞系ｍｄｒｌ基因表达。结果显示Ｋ５６２／ＶＣＲ有较高水平ｍｄｒｌ基因表达（０．８６），而Ｋ５６２／Ｓ未检测到表达。此外，利用酶学方法和点杂交方法在蛋白质和ｍＲＮＡ水平上检测了Ｋ５６２／Ｓ和Ｋ５６２／ＶＣＲ中谷胱甘肽Ｓ转移酶（ＧＳＴ）活性及其基因表达，发现Ｋ５６２／ＶＣＲ中ＧＳＴ活性明显高于Ｋ５６２／Ｓ（Ｐ＜０．００５），且该种活性的增高是由ＧＳＴπ、ＧＳＴμ过度表达引起。
sensitive,specific and quantitative RT-PCR methodfor detecting the expression of mdr 1 mRNA was estab-lished. The expression of mdr 1 mRNA in sensitive cellline K562 and multidrug resistant cell line K562/VCRwere assayed. The expression of mdr 1 mRNA was de-tected with a high level ( 0. 86) in K562/VCR but unde-tectable in K562 (PCR 30 cycles ). The expression ofGST gene in K562 and K562 /VCR was assayed at pro-tein and mRNA levels by enzymological method and dotblot. The results showed that GST level was higher inK562/VCR than in K562 (P< 0. 005 ) and this higherlevel in K562/VCR was due to the overexpression ofGSTμand GSTπ.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1995年第4期193-195,共3页
Chinese Journal of Hematology
基金
天津市21世纪青年科学基金
