三株体外长期培养的骨髓造血细胞系的建立及其生物学特性研究

ESTABLISHMENT AND CHARACTERISTICS OF THREEHUMAN HEMATOPOfETIC CELL LINES

用长期骨髓培养（ＬＴＢＭＣ）的方法培养了１２例急性白血病患者和５例正常人骨髓，获得三株能在体外增殖的造血细胞株（ＸＧ＿（２９）、ＸＧ＿（３４）、ＸＧ＿（３９）），它们均为ＣＤ＿（３４）。其中，ＸＧ＿（３９）在ＩＬ－１／ＩＬ－６刺激下增加Ｂ淋巴细胞抗原表达，倍增时间缩短；在Ｇ－ＣＳＦ／ＧＭ－ＣＳＦ刺激下抑制Ｂ淋巴细胞抗原表达，倍增时间延长。另外，ＸＧ＿（２９）经培养核型由异常转为正常，ＸＧ＿（２９）、ＸＧ＿（３９）亦大量分泌肿瘤坏死因子α结果提示：ＬＴＢＭＣ可以使急性白血病骨髓中的白血病细胞染色体核型由异常转变为正常，而且该三株细胞对研究造血细胞的分化发育具有重要价值。

y using a long-term bone marrow culture(LTBMC) systern , three CD_(34) positive hematopoieticcell lines (XG_(29) , XG_(34) , XG_(39) were established from 12cases of acute leukemia (AL) and 5 normal donors.Under the stimulation of IL-I/IL-6, the expression ofBlymphocyte antigen was increased and the doubling-time shortened in the XG_(39) cells. On the contrary, theexpression of Blymphocyte antigen decreased and thedoubling-time prolonged in the XG_(39) cells stimulated byG-CSF/GM-CSF. Moreover, the abnormal karyotypeof XGzo cell turned riormal and the XG_(39)and XG_(29) cellssecreted a large amount of TNFa through LTBMC.The results suggested that the leukemia cells in thebone marrow might be eradicated through LTBMC,and these three cell lines could be valuable tools forstudying the differentiation and proliferation ofhematopoietic cells.