IL－6和GM－CSF对人多发性骨髓瘤细胞与血管内皮细胞粘附效应作用的研究

EFFECT OF IL-6 AND GM-CSF ON THE ADHESIVEINTERACTION BETWEEN HUMAN MYELOMA CELLSAND VASCULAR ENDOTHELIAL CELLS

用￣（５１）Ｃｒ标记细胞株的方法，研究了白细胞介素６（ＩＬ－６）及粒－巨噬系集落刺激因子（ＧＭ－ＣＳＦ）对人多发性骨髓瘤（ＭＭ）细胞株（Ｕ２６６、ＸＧ－７）、ＥＢ病毒阳性细胞株（Ｄａｕｄｉ）和内皮细胞间粘附的调控作用，观察了粘附作用对ＸＧ－７细胞表面粘附分子表达的影响。结果表明：①ＩＬ－６及其受体（ＩＬ－６Ｒ）ｇｐ１３０相关性生长因子ＧＭ－ＣＳＦ不仅是人ＭＭ细胞体内、外的生长因子，而且可以提高ＭＭ细胞－内皮细胞间的粘附能力，有助于ＭＭ细胞的扩散；②粘附作用亦能引起肿瘤细胞ＸＧ－７表面ＣＤ＿（１１ａ）、ＣＤ＿（５４）、ＣＤ＿（４４）、ＣＤ＿（４９ｄ）表达的异常改变；③ＣＤ＿（４９ｄ）及其配体的相互作用可能参与了ＭＭ细胞与内皮细胞的粘附过程。

he regulating effects of IL-6 and GM-CSF onthe adhesive interaction between myeloma cell lines(U_(266), XG-7) IEBV￣+ cell line(Daudi) and the endothe-lial cells were studied, and the effect of adhesive inter-action on the adhesive molecule expression of XG-7was also examined. The results showed that ① IL-6and IL-6R gp130 associated factors are not only thegrowth factors for myeloma cells, but also factors re-lated to the metastasis of the tumor cells. ② The ad-hesive interaction resulted in changes of adhesivemolecule (CD_(11a) , CD_(54) , CD_(44) and CD_(49d)) expression onXG-7 cells. ③ CD_(49d) may be one of the molecules in-volved in adhering process of U_(266)/XG-7 and endothe-lial cells.