应用序列特异引物PCR法对广西壮族HLA－DQA_1进行基因分型

HLA-DQA1 GENOTYPING FOR GUANGXI ZHUANGNATIONALITY BY THE PCR-SSP TECHNIQUE

应用序列特异引物多聚酶链反应（ＰＣＲ）方法对８２名无血缘关系的广西壮族健康人的ＨＬＡ－ＤＱＡ＿１进行基因分型。共检出７种ＤＱＡ＿１等位基因，以ＤＱＡ＿１＊０３０１的频率最高，达３１％，其次为ＤＱＡ＿１＊０１０４，０１０２和０５０１，检出率分别为２４．３％，１６．９％和１１．７％。未检出的等位基因有ＤＱＡ＿１＊０２０１，０３０２和０６０１。结果表明，壮族的ＨＬＡ－ＤＱＡ＿１等位基因频率分布除与中国南方人相似外，尚有其特点。序列特异引物ＰＣＲ方法不需要放射性同位素，简便快速、准确可靠的ＨＬＡ－ＤＱＡ＿１基因分型新方法。

LA typing for DQA1 locus in 82 unrelated,ran-domly selected,healthy individuals of Guangxi ZhuangNationality was carried out.Seven officially recognizedalleles were detected,of which DQA1 * 0301 occurdwith the highest frequency of 3 1%,Other alleles ofhigh frequency included DQAi * 0104 ,0102 and 0501with frequencies of 24.3%, 16.9%and 11.7%,re-spectively, However, DQA_1 * 0201, 0302 and 0601were not detected.Our results indicated that the HLA-DQA_1 of Zhuang Nationality were not only similar tothose of southern Chinese populations,but also exhib-ited their own unique features. PCR-SSP technique is anon-radioactive,accurate and reliable method for HLAgenotyping.