自体LAK细胞对白血病细胞清除作用的实验研究

STUDY ON THE CYTOTOXICITY OF AUTOLOGOUSLAK CELLS TO FRESHLEUKEMIA CELLS

采用￣（５１）Ｃｒ４小时释放试验，细胞培养及组化分析技术，研究了２６例初诊急性髓细胞白血病患者（ＡＰｓ）和其中的１２例缓解期患者（ＲＰｓ）的ＬＡＫ细胞对自体白血病细胞（Ａｕｔｏ－ＬＢ）的清除作用。ＡＰｓ的外周血或骨髓通过双层Ｆｉｃｏｌｌ－Ｈｙｐａｑｕｅ分离所得富含淋巴细胞层中含２０％～５０％的Ａｕｔｏ－ＬＢ，在ｒＩＬ－２１０００Ｕ／ｍｌ的培养基中培养１～２周Ａｕｔｏ－ＬＢ消失。而￣（５１）Ｃｒ４小时释放试验的结果是ＡＰｓ－ＬＡＫ和ＲＰｓ－ＬＡＫ细胞胞对Ａｕｔｏ－ＬＢ的杀伤活性分别为１２．９％±１２．３％和２４．１％±１２．０％，均较其对Ｒａｊｉ细胞的杀伤活性（３６．０％±３２．０％和６６．２％±１８．１％）显著减低（Ｐ＜０．０１），提示：Ａｕｔｏ－ＬＢ细胞表面能被ＬＡＫ细胞识别的决定簇较Ｒａｊｉ细胞系低。但体外培养过程中Ａｕｔｏ－ＬＢ能被清除。提示：清除机制除了有ＬＡＫ细胞的直接杀伤外，尚有其它机制参与，体外培养过程中Ａｕｔｏ－ＬＢ表面决定簇表达增加。

ith ￣(51)Cr 4-hour release assay, cell culture,andhistochemistry technique,the cytotoxicity of freshleukemia cells induced with autologous LAK cells from26 patients with newly diagnosed acute myelogenousleukemia(APs)and 12 patients in remission ( RPs )were studied.The cell layer enriched with lymphocytescontained 2O%～50% of leukemia cells. They were de-stroyed during the coculture with rIL-2 at 1000U/mlfor 1～2 weeks,The result of ￣(51)Cr 4-hour release as-say indicated that the cytotoxicity of APs-LAK andRPs-LAK cells to Auto-LB were 12.9%±12.3% and24.1%±12.0%,respectively, being lower statistical-ly than that of RPs-LAK and APs-LAK cells to Rajicell line(36.O%±32.O% and 66.2%±18.1%)(P<0. 01 ).It suggested that the mechanism of leukemicblasts being destroyed during the coculture in vitrowith rIL-2 was involved in several aspects, and short-term coculture of leukemic blasts could increase thesusceptibility to autologous LAK cells.