胰岛素样生长因子Ⅰ基因在正常及糖尿病鼠眼组织中的表达

Expression of insulin-like growth factor-Ⅰ gene in normal and diabetic rat eye

用原位杂交技术对正常及糖尿病大鼠眼组织的胰岛素样生长因子Ⅰ基因（ｉｎｓｕｌｉｎ－ｌｉｋｅｇｒｏｗｔｈｆａｃｔｏｒ－Ⅰ，ＩＧＦ－Ⅰ）进行了研究。ＩＧＦ－Ⅰ基因在眼组织的表达呈区域性，视网膜内核层及神经节细胞表达最强；脉络膜、视网膜色素上皮细胞及外核层次之；巩膜最弱，角膜未见表达信号。糖尿病眼组织ＩＧＦ－Ⅰ基因表达显著增强（Ｐ＜０．０５或Ｐ≤０．０１）。结果表明：整个眼球组织形成了一个ＩＧＦ－Ⅰ旁分泌、自分泌作用系统；糖尿病眼组织ＩＧＦ－Ⅰ基因高表达预示ＩＧＦ－Ⅰ可诱发并加速糖尿病视网膜病变的发生发展。

The expression of insulin-like growth factor Ⅰ(IGF-Ⅰ)gene was studied in both normaland diabetic rat eyes via in situ hybridization. The results revealed the regional expression of IGF-Ⅰgenein the rat eyes. The expression of IGF-Ⅰ mRNA in the internal nuclear layer and ganglion cell layer isstrong, in choroid, retinal pigment epithelium and external nuclear layer is moderate, in sclera is weakin degree a nd no such mRNA is detected in the cornea. The abundance of IGF-Ⅰ mRNA in diabetic eyetissues is significantly(P< 0. 05 or P≤0.01 ) higher than that in normal eye. These findings suggestthat(1) functionally, the eye ball be considered to be an “IGF-Ⅰ paracrine-autocrine system”, and(2)the high expression of IGF-Ⅰ indicate its initiation of diabetic retinopathy and its promotion of the pro-gression of the lesion.