IL－2－PE40对异位角膜植入抗排斥反应的研究

Au experimental study of corueal allograft rejection with cytotoxin IL-2- PE40

采用新型免疫抑制剂白细胞介素－２－绿脓杆茵外毒素（ｉｎｔｅｒｌｅｕｋｉｎ－２－Ｐｓｅｕｄｏｍｏｎａｓｅｘｏｔｏｘｉｎ４０，ＩＬ－２－ＰＥ４０）对角膜移植排斥反应进行实验性治疗。以Ｗｉｓｔａｒ大鼠异种角膜异位移植为模型，将３６只鼠随机分为治疗组及对照组，并在术后第２、５、７、１０、１４天分别取出植片做病理切片，收集外周血进行Ｔ淋巴细胞亚群及Ｔ淋巴细胞集落形成数分析。结果显示：ＩＬ－２－ＰＥ４０可推迟排斥反应的发生时间；有明显减少外周血中辅助Ｔ淋巴细胞百分率的作用；并能减弱大鼠外周血Ｔ淋巴细胞巢落形成能力。

We used a new immunosuppressive agent interleukin( IL )-2-Pseudomonas exotoxin 40(PE40 ) to treat corneal allograft rejection,using the Wistar rat model of heterograft-heterotopic graft rejection, 36 rats were divided randomly into the treatment and the control groups. On 2,5,7, 10 and 14 days after the transplantation,the transplanted corneas were removed for pathological examinations. The peripheral blood was collected for the analysis of T cell subgroups and T lymphocyte colony forming unit(T- CFU) assay,These results indicate that IL-2-PE40 can delay the development of corneal graft rejection and significantly reduce the percentage of T-helper cells. On the other hand,IL-2-PE40 can weaken the gathering capacity of the peripheral T- cells.