摘要
为了早期诊断风疹感染，应用逆转录。聚合酶链反应（ＲＴ－ＰＣＲ）方法对８０例临床怀疑风疹感染患者的咽拭物检测风疹病毒。ＰＣＲ阳性者４３例，占５３％；同时用酶联免疫吸附法对每份血清作了ＩｇＭ检测，阳性者１２例，占１５％。统计学处理（Ｐ＜０．０１），表明两种检测方法差异有显著性。扩增产物的特异性通过地高辛标记探针的点杂交分析得到证实。该ＲＴ－ＰＣＲ方法具有快速、早期、灵敏度高和特异性强的特点，有希望发展成为临床诊断胎儿风疹感染的一种重要手段，并为产前咨询提供可靠的依据。
he reverse transcription and nested polymerase chain reaction(RT-PCR )was used to detect thespecific genomic fragment of rubella virus in 80 nasopharyngeal secretion specimens from the suspectedrubella virus infected patients. At the same time,ELISA for detecting the specific serum IgM was ap-plied. Results showed that positive rate was 5 3%by RT-PCR and 1 5%by ELISA. The difference ofthe positive rate between the two assays was statistically significant(P< 0.01). The specificity of theamplified fragment was confirmed by the dot blot hybridization with a Digoxigenin labeled probe. ThisRT-PCR assay provides a rapid, sensitive and specific test for the diagnosis of rubella virus infection.Because rubella virus is one of the important teratogenic viruses, it could be invaluable in providing pre-natal counselling following rubella virus infection during early pregnancy.
