摘要
为探讨自然杀伤细胞（ＮＫ）活性测定在癌症诊治中的作用及机理，采用￣３Ｎ－ＴｄＲ掺入抑制法，分别对５８例癌症及２０例良性病变患者手术或免疫治疗前后的ＮＫ活性进行了动态比较分析，结果提示：结直肠良性病变组术前（４０．１５％±１０．７８％）、术后（３９．６８％±１３．６３％），与正常对照组（４５．９０％±１６．２０％）相比差异无显著意义（Ｐ＞０．０５）。胃良性病变组术前（３０．７１％±８．４０％）明显低于正常对照组，术后（４５．６０％±２１．７０％）恢复正常。胃癌组术前（２９．６０％±１３．７０％）与结直肠癌组术前（２６．７７％±１４．１４％）均明显低于正常对照组（Ｐ＜０．０１），术后胃癌组（３３．２０±１６．００％）和结直肠癌组（３４．８６％±１６．８８％）均可见回升，但尚未恢复正常（Ｐ＜０．０５）。直结肠癌干扰素－α治疗组，用药前（２４．３１％±１１．６２％）与用药后（３９．９５％±７．４７％）差异显著（Ｐ＜０．０５）。淋巴因子激活性杀伤细胞或肿瘤浸润淋巴细胞／白细胞介素２晚期癌症治疗组，治疗前显著降低，治疗后１００％病例呈上升趋势，其中５３％病例恢复正常，并与抗癌疗效呈正比。结果表明ＮＫ活性检测是一项理想的?
In order tO study the mechanism and effect of NK cells in cancer patients a ￣3H-TdR incorporation suppressive method was used for dynamic comparison and analysis of NK cell activity in 58 cancer pa-tients and 20 patients with benign lesions before and after operation and immunotherapy. The results showed that the activities before(40. 15%±10. 78%)and after operation (36. 68%±13.63%)in col-orectal simple lesion group did not markedly differ from that in the normal control(45. 90%±16. 20%)(P> 0.05 ). In gastric simple lesion group,the preoperative activity(30.71%±8. 40%)was much lower than that in the normal control.It restored to normal value after operation(45.60%±21.70%). The preoperative values in gastric carcinoma group(29. 60%±13.70%).and colorectal cancer group(26.77%±14. 14%)were low as compared to the normal control(P<0. 01).After operation the val-ue in gastric cancer group(33.20%±16. 00%)and colorectal cancer group(34. 86%±16.88%)in-crease but still below the normal value (P<0. 05).The outcome before(24. 31%±11. 62%)and after medication or α-interferon(39.95%±7.47%)in colorectal cancer group showed significant difference(P<0.05).In LAK or TIL/IL-2 treating advanced malignancy group, it conspicuously decreased be-fore the treatment, but after the treatment a tendency to rise activities showed in 100%patients,being directly proportional to the anticancer effectiveness. Of them,53%of the patients vegained normal val-ue. All results demonstrate that NK cell activity assav is a valuable indication for immune survev of can-cers,which will assist in making diagnosis and selection of anticancerous regimes.
基金
江苏省科学委员会
